Titration of microorganisms in infectious or environmental samples is a corner stone of quantitative microbiology. A simple method is presented to estimate the microbial counts obtained with the serial dilution technique for microorganisms that can grow on bacteriological media and develop into a colony. Dilution di-loo´shun 1. Reduction of concentration of an active substance by admixture of a neutral agent. A substance that has undergone such a process. Serial dilution a set of dilutions in a mathematical sequence. In microbiological technique, serial dilutions are used to obtain a culture plate that yields a countable number of separate. Your best bet is a serial dilution, i.e. Get 2 10ml volumetric flasks and take 1ml of your solution to be diluted, and make to 10ml with solvent, mix this, then take 1ml of the resulting 10mls. When calculating dilution factors, it is important that the units of volume and concentration remain consistent. Dilution calculations can be performed using the formula M 1 V 1 = M 2 V 2. A serial dilution is a series of stepwise dilutions, where the dilution factor is held constant at each step. Oct 08, 2013 A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration. The easiest method is to make a series of 1 in 10 dilutions.
Also found in: Dictionary, Thesaurus, Financial, Encyclopedia. dilution
[di-loo´shun]Serial Dilution Technique Lab Report
1. reduction of concentration of an active substance by admixture of a neutral agent.
serial dilution a set of dilutions in a mathematical sequence. In microbiological technique, serial dilutions are used to obtain a culture plate that yields a countable number of separate colonies. From this, a calculation of viable cells in the original suspension can be made, as a colony picked for pure culture.
se·ri·al di·lu·tion
(sēr'ē-ăl di-lū'shŭn)The preparation of a graded set of solutions or suspensions, each member of the set being a fixed dilution (typically 1:2) of the preceding member; used particularly in titration of antibodies in serum.
Want to thank TFD for its existence? Tell a friend about us, add a link to this page, or visit the webmaster's page for free fun content.
Link to this page: